The choice of primary recovery technique or operation of bioconversion had no effect on the column performance. This was successfully operated in a packed bed column producing an effective extraction and concentration step. Physical adsorption was achieved by the use of the resin Amberlite XAD-4. The effects of process stream and choice of catalyst recovery technique on these concentration techniques has been studied. Only the use of physical adsorption and liquid-liquid extraction proved effective. Techniques applicable to the product concentration of the lactone products have been assessed. Catalyst recovery was investigated by use of centrifugation and both micro- and ultrafiltration. Extraction of products was studied direct from the bioconversion in spent fermentation media and resuspended catalyst in phosphate buffer. This is achieved by the use of an Escherichia coli TOP 10 whole cell catalyst. The model reaction for study is the commercially important bioconversion of racemic ketone bicyclohept-2-ene-6-one to the lactone products (1S,5R)-2-oxabicyclooct-6-en-3-one and (1R,5S)-3-oxabicyclo-oct-6-en-2-one. This work aims to study the options for catalyst recovery and product concentration from a whole cell bioconversion. The overall process route to the purification of bioconversion products may be described in three parts: catalyst removal, product concentration and product isolation. This equipment is designed to deliver multi-kilo quantities of material, but of course we have a small scale version for your process development.Downstream Processing from Baeyer-Villiger Monooxygenase Bioconversions.ĭoctoral thesis (Ph.D), UCL (University College London).Īlthough much work has been carried out into the study of bioconversions and the associated upstream process options, very little has focused on the issues of downstream product recovery. We have tapped into the inherently excellent control provided by continuous crystallisation technology to develop our Rattlesnake Continuous Crystallisation Concept. These state-of-the-art platforms collect all the data needed to deliver understanding of your downstream processes by combining real-time machine vision with robotics, performing repetitive tasks, day and night, with high levels of precision and accuracy.Ĭrystallisation perhaps poses the greatest challenge in process control. To help you gain full process understanding of your filtration/washing and liquid-liquid extraction unit operations and avoid frustrating scale-up issues, CRD has designed and engineered the Falcon Filtration and the Eagle Extraction Robot Assistants.
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